Characterization and comparison of human limbal explant cultures grown under defined and xeno-free conditions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10382997" target="_blank" >RIV/00216208:11110/18:10382997 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/18:43916759 RIV/00064173:_____/18:N0000001 RIV/00064165:_____/18:10382997
Result on the web
<a href="https://doi.org/10.1016/j.exer.2018.06.019" target="_blank" >https://doi.org/10.1016/j.exer.2018.06.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exer.2018.06.019" target="_blank" >10.1016/j.exer.2018.06.019</a>
Alternative languages
Result language
angličtina
Original language name
Characterization and comparison of human limbal explant cultures grown under defined and xeno-free conditions
Original language description
Human limbal epithelial cells (LECs) intended for treatment of limbal stem cell deficiency are commonly cultivated on a 3T3 feeder layer with complex culture medium supplemented with fetal bovine serum (FBS). However, FBS is a xenogeneic component containing poorly characterised constituents and exhibits quantitative and qualitative lot-to-lot variations. Human limbal explants were plated on untreated or fibrin coated plastic plates and cultured in two non-xenogeneic media (supplemented with either human serum or platelet lysate only). Our aim was to find out whether the characteristics of harvested LEC cultures are comparable to those of LEC cultivated in the gold standard - FBS-supplemented complex medium. The growth kinetics, cell proliferation, differentiation, sternness maintenance, apoptosis and contamination by other cell types were evaluated and compared among these conditions. In all of them LECs were successfully cultivated. Sternness was preserved in both xeno-free media. However, cells cultured with human serum on the fibrin-coated plates had the highest growth rate and cell proliferation and very low fibroblast-like cell contamination. These data suggest that xenofree cell culture conditions can replace the traditional FBS-supplemented medium and thereby provide a safer protocol for ex vivo cultured limbal stem cell transplants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30207 - Ophthalmology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental Eye Research
ISSN
0014-4835
e-ISSN
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Volume of the periodical
176
Issue of the periodical within the volume
November
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
20-28
UT code for WoS article
000449902000003
EID of the result in the Scopus database
2-s2.0-85049097445