Electrospun poly(L-lactide-co-DL-lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F24%3A43927086" target="_blank" >RIV/00064173:_____/24:43927086 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/24:00586028 RIV/00216208:11110/24:10480836 RIV/00216208:11120/24:43927086
Result on the web
<a href="https://doi.org/10.1016/j.heliyon.2024.e30970" target="_blank" >https://doi.org/10.1016/j.heliyon.2024.e30970</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.heliyon.2024.e30970" target="_blank" >10.1016/j.heliyon.2024.e30970</a>

Result language
angličtina
Original language name
Electrospun poly(L-lactide-co-DL-lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation
Original language description
Ultrathin electrospun poly (L-lactide-co-DL-lactide) nanofibrous membranes coated with fibronectin were explored as scaffolds for the ex vivo cultivation of limbal epithelial cells (LECs) for the treatment of limbal stem cell deficiency. The developed scaffolds were compared with the "gold-standard" fibrin gel. The resulting membranes composed of nanofibers possessed a very low thickness of 4 μm and allowed very good optical transparency in the wet state. The fibronectin-coated nanofibrous scaffolds demonstrated LEC expansion and successful cultivation similar to that on fibrin gel. Unlike the regular cobblestone epithelial cell morphology on the fibrin gel, the nanofibrous scaffold presented a mostly irregular epithelial morphology with a shift to a mesenchymal phenotype, as confirmed by the upregulation of profibroblastic genes: ACTA2 (p = 0.023), FBLN1 (p < 0.001), and THY1 (p < 0.001). Both culture conditions revealed comparable expression of stem cell markers, including KLF4, ΔNp63α and ABCG2, emphasizing the promise of polylactide-based nanofibrous membranes for further investigations.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30207 - Ophthalmology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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