Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00474274" target="_blank" >RIV/67985823:_____/17:00474274 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/17:00474274 RIV/67985891:_____/17:00474274 RIV/00216208:11130/17:10360964 RIV/00216208:11510/17:10360964
Result on the web
<a href="http://dx.doi.org/10.2147/IJN.S121299" target="_blank" >http://dx.doi.org/10.2147/IJN.S121299</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2147/IJN.S121299" target="_blank" >10.2147/IJN.S121299</a>
Alternative languages
Result language
angličtina
Original language name
Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
Original language description
Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Result continuities
Project
<a href="/en/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Preparation, modification and characterization of materials by radiation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Nanomedicine
ISSN
1178-2013
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
Feb
Country of publishing house
NZ - NEW ZEALAND
Number of pages
18
Pages from-to
1143-1160
UT code for WoS article
000393596400001
EID of the result in the Scopus database
2-s2.0-85012286996