The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985891%3A_____%2F16%3A00459829" target="_blank" >RIV/67985891:_____/16:00459829 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/16:00459829 RIV/61389013:_____/16:00459829 RIV/00216208:11130/16:10323709

Result on the web

<a href="http://dx.doi.org/10.2147/IJN.S99317" target="_blank" >http://dx.doi.org/10.2147/IJN.S99317</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S99317" target="_blank" >10.2147/IJN.S99317</a>

Result language

angličtina

Original language name

The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

Original language description

Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly(L-lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta1-integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JI - Composite materials

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

2016

Country of publishing house

NZ - NEW ZEALAND

Number of pages

19

Pages from-to

771-789

UT code for WoS article

000370931600001

EID of the result in the Scopus database

2-s2.0-84959419065