Circulating Tumor Cells as an Auxiliary Diagnostic Tool in Surgery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10365065" target="_blank" >RIV/00216208:11110/17:10365065 - isvavai.cz</a>

Alternative codes found

RIV/00064173:_____/17:N0000043 RIV/00064203:_____/17:10365065

Result on the web

<a href="http://dx.doi.org/10.21873/invivo.11190" target="_blank" >http://dx.doi.org/10.21873/invivo.11190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21873/invivo.11190" target="_blank" >10.21873/invivo.11190</a>

Result language

angličtina

Original language name

Circulating Tumor Cells as an Auxiliary Diagnostic Tool in Surgery

Original language description

Background: In general, the presence of circulating tumor cells (CTCs) in peripheral blood (PB) is associated with a relative shorter overall survival in cancer patients. The clinical utility of CTC diagnostics is changing: from prognostic test to an assay predicting therapy response, enabling the right choice of therapy and monitoring the effect of administered therapy. We present two case reports of patients with suspicion of lung and pancreatic cancer, without obtainable preoperative biopsy for histological verification. The focus of the presented study was not to deliver a complete tumor tissue classification to the surgeon, but to answer the question if there is malignant disease or not. The results are based on CTC presence and characterization. Materials and Methods: A size-based separation method for viable CTC enrichment from anticoagulated PB was used. The separated cells were cytomorphologically examined using vital fluorescent microscopy. Additionally, to confirm the epithelial origin of the cells on the separation membrane, CTC gene expression analysis was performed. Results: CTCs were successfully enriched and cultured in vitro in both tested samples. The epithelial character of the captured cells was confirmed by quantitative-polymerase chain reaction (qPCR) analysis for a set of tumor-associated genes. Conclusion: Detection of cancer cells in PB (liquid biopsy) and their molecular characterization could significantly help complete the tumor diagnostic process in a time-efficient manner.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

In Vivo

ISSN

0258-851X

e-ISSN

—

Volume of the periodical

31

Issue of the periodical within the volume

6

Country of publishing house

GR - GREECE

Number of pages

6

Pages from-to

1197-1202

UT code for WoS article

000414546400023

EID of the result in the Scopus database

2-s2.0-85033489317