Biogenesis of Hydrogen Sulfide and Thioethers by Cystathionine Beta-Synthase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10366186" target="_blank" >RIV/00216208:11110/18:10366186 - isvavai.cz</a>
Alternative codes found
RIV/00064165:_____/18:10366186
Result on the web
<a href="http://dx.doi.org/10.1089/ars.2017.7009" target="_blank" >http://dx.doi.org/10.1089/ars.2017.7009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/ars.2017.7009" target="_blank" >10.1089/ars.2017.7009</a>
Alternative languages
Result language
angličtina
Original language name
Biogenesis of Hydrogen Sulfide and Thioethers by Cystathionine Beta-Synthase
Original language description
Aims: The transsulfuration pathway enzymes cystathionine beta-synthase (CBS) and cystathionine gamma-lyase are thought to be the major source of hydrogen sulfide (H2S). In this study, we assessed the role of CBS in H2S biogenesis. Results: We show that despite discouraging enzyme kinetics of alternative H2S-producing reactions utilizing cysteine compared with the canonical condensation of serine and homocysteine, our simulations of substrate competitions at biologically relevant conditions suggest that cysteine is able to partially compete with serine on CBS, thus leading to generation of appreciable amounts of H2S. The leading H2S-producing reaction is condensation of cysteine with homocysteine, while cysteine desulfuration plays a dominant role when cysteine is more abundant than serine and homocysteine is limited. We found that the serine-to-cysteine ratio is the main determinant of CBS H2S productivity. Abundance of cysteine over serine, for example, in plasma, allowed for up to 43% of CBS activity being responsible for H2S production, while excess of serine typical for intracellular levels effectively limited such activity to less than 1.5%. CBS also produced lanthionine from serine and cysteine and a third of lanthionine coming from condensation of two cysteines contributed to the H2S pool. Innovation: Our study characterizes the H2S-producing potential of CBS under biologically relevant conditions and highlights the serine-to-cysteine ratio as the main determinant of H2S production by CBS in vivo. Conclusion: Our data clarify the function of CBS in H2S biogenesis and the role of thioethers as surrogate H2S markers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/NV16-30384A" target="_blank" >NV16-30384A: Metabolism of organic and inorganic sulfur compounds in selected human diseases</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Antioxidants & Redox Signaling
ISSN
1523-0864
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
311-323
UT code for WoS article
000414560200001
EID of the result in the Scopus database
2-s2.0-85039869142