Note on the use of different approaches to determine the pore sizes of tissue engineering scaffolds: what do we measure?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10378665" target="_blank" >RIV/00216208:11110/18:10378665 - isvavai.cz</a>

Alternative codes found

RIV/67985891:_____/18:00497416 RIV/68407700:21220/18:00329402 RIV/00064165:_____/18:10378665

Result on the web

<a href="https://doi.org/10.1186/s12938-018-0543-z" target="_blank" >https://doi.org/10.1186/s12938-018-0543-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12938-018-0543-z" target="_blank" >10.1186/s12938-018-0543-z</a>

Result language

angličtina

Original language name

Note on the use of different approaches to determine the pore sizes of tissue engineering scaffolds: what do we measure?

Original language description

Background: Collagen-based scaffolds provide a promising option for the treatment of bone defects. One of the key parameters of such scaffolds consists of porosity, including pore size. However, to date, no agreement has been found with respect to the methodology for pore size evaluation. Since the determination of the exact pore size value is not possible, the comparison of the various methods applied is complicated. Hence, this study focuses on the comparison of two widely-used methods for the characterization of porosity-scanning electron microscopy (SEM) and micro-computed tomography (micro-CT). Methods: 7 types of collagen-based composite scaffold models were prepared by means of lyophilization and collagen cross-linking. Micro-CT analysis was performed in 3D and in 2D (pore size parameters were: major diameter, mean thickness, biggest inner circle diameter and area-equivalent circle diameter). Afterwards, pore sizes were analyzed in the same specimens by an image analysis of SEM microphotographs. The results were statistically evaluated. The comparison of the various approaches to the evaluation of pore size was based on coefficients of variance and the semi-quantitative assessment of selected qualities (e.g. the potential for direct 3D analysis, whole specimen analysis, non-destructivity). Results: The pore size values differed significantly with respect to the parameters applied. Median values of pore size values were ranging from 20 to 490 mu m. The SEM values were approximately 3 times higher than micro-CT 3D values for each specimen. The Mean thickness was the most advantageous micro-CT 2D approach. Coefficient of variance revealed no differences among pore size parameters (except major diameter). The semi-quantitative comparison approach presented pore size parameters in descending order with regard to the advantages thereof as follows: (1) micro-CT 3D, (2) mean thickness and SEM, (3) biggest inner circle diameter, major diameter and area equivalent circle diameter. Conclusion: The results indicated that micro-CT 3D evaluation provides the most beneficial overall approach. Micro-CT 2D analysis (mean thickness) is advantageous in terms of its time efficacy. SEM is still considered as gold standard for its widespread use and high resolution. However, exact comparison of pore size analysis in scaffold materials remains a challenge.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BioMedical Engineering Online

ISSN

1475-925X

e-ISSN

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Volume of the periodical

17

Issue of the periodical within the volume

August

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000442046900001

EID of the result in the Scopus database

2-s2.0-85051958639