Interaction of a Potential Anticancer Agent Hypericin and its Model Compound Emodin with DNA and Bovine Serum Albumin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10379667" target="_blank" >RIV/00216208:11110/18:10379667 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.21873/invivo.11347" target="_blank" >https://doi.org/10.21873/invivo.11347</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21873/invivo.11347" target="_blank" >10.21873/invivo.11347</a>
Alternative languages
Result language
angličtina
Original language name
Interaction of a Potential Anticancer Agent Hypericin and its Model Compound Emodin with DNA and Bovine Serum Albumin
Original language description
Background/Aim: We report the incorporation of prospective anticancer agent hypericin into DNA and bovine serum albumin (BSA), respectively, with emphasis on comparison of the differences in interaction mode between hypericin and its model compound emodin. Materials and Methods: Spectrophotometric methods were used for determination of the binding constants of the drug complex with biomacromolecules. Differential scanning calorimetry was applied for evaluation of drug-macromolecule complex thermal stability. Results: The strength of interaction expressed by binding constants was found to be 4.0x10(4) l/mol for hypericin-DNA and 8.1x10(4) l/mol for emodin-DNA complex. Both molecules stabilize bovine serum albumin macromolecule and bind into the hydrophobic cavity in IIA subunit but their localization within the molecule is different. Conclusion: Anticancer agent hypericin and its derivative emodin interact with DNA with medium strength and are probably incorporated into the groove of DNA by hydrogen bonds. Bovine serum albumin can serve as a transport protein for hypericin since the binding force between both molecules is adequate.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
In Vivo
ISSN
0258-851X
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
5
Country of publishing house
GR - GREECE
Number of pages
8
Pages from-to
1063-1070
UT code for WoS article
000442807900010
EID of the result in the Scopus database
2-s2.0-85053040911