Chiroptical Properties of Bilirubin-Serum Albumin Binding Sites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F13%3A43895072" target="_blank" >RIV/60461373:22340/13:43895072 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/chir.22143" target="_blank" >http://dx.doi.org/10.1002/chir.22143</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chir.22143" target="_blank" >10.1002/chir.22143</a>
Alternative languages
Result language
angličtina
Original language name
Chiroptical Properties of Bilirubin-Serum Albumin Binding Sites
Original language description
Although the interactions between bilirubin and serum albumin are among the most studied serum albumin-ligand interactions, the binding-site location and the participation of bilirubin-serum albumin complexes in pathological and physiological processes are under debate. In this article, we have benefited from the chiral structure of bilirubin and used CD spectroscopy to characterize the structure of bilirubin bound to human and bovine serum albumins. We determined that in a phosphate buffer at pH7.8 there are three binding sites in both human and bovine serum albumins. While the primary binding sites in human and bovine serum albumins bind bilirubin with P- and M-helical conformations, respectively, the secondary binding sites in both albumins bind bilirubin in the P-helical conformation. We have shown that the bonding of bilirubin to the serum albumin matrix is a more favorable process than the self-association of bilirubin under the studied conditions, with a maximum of three bound b
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP206%2F11%2F0836" target="_blank" >GAP206/11/0836: Structural Study of Potentially Bioactive Bile Pigment Complexes: Relation to their Protective Function in Organisms</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chirality
ISSN
0899-0042
e-ISSN
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Volume of the periodical
25
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
257-263
UT code for WoS article
000316913100007
EID of the result in the Scopus database
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