Circular dichroism study of the interaction between mutagens and bilirubin bound to different binding sites of serum albumins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F14%3A43897030" target="_blank" >RIV/60461373:22340/14:43897030 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.saa.2014.01.139" target="_blank" >http://dx.doi.org/10.1016/j.saa.2014.01.139</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.saa.2014.01.139" target="_blank" >10.1016/j.saa.2014.01.139</a>

Result language

angličtina

Original language name

Circular dichroism study of the interaction between mutagens and bilirubin bound to different binding sites of serum albumins

Original language description

Although recent investigations have shown that bilirubin not only has a negative role in the organism but also exhibits significant antimutagenic properties, the mechanisms of interactions between bilirubin and mutagens are not clear. In this study, interaction between bilirubin bound to different binding sites of mammalian serum albumins with structural analogues of the mutagens 2-aminofluorene, 2,7-diaminofluorene and mutagen 2,4,7-trinitrofluorenone were investigated by circular dichroism and absorption spectroscopy. Homological human and bovine serum albumins were used as chiral matrices, which preferentially bind different conformers of bilirubin in the primary binding sites and make it observable by circular dichroism. These molecular systems approximated a real system for the study of mutagens in blood serum. Differences between the interaction of bilirubin bound to primary and to secondary binding sites of serum albumins with mutagens were shown. For bilirubin bound to secondar

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GAP206%2F11%2F0836" target="_blank" >GAP206/11/0836: Structural Study of Potentially Bioactive Bile Pigment Complexes: Relation to their Protective Function in Organisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

ISSN

1386-1425

e-ISSN

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Volume of the periodical

126

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

68-75

UT code for WoS article

000335618200010

EID of the result in the Scopus database

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