Ethanol fixation method for heart and lung imaging in micro-CT
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F19%3A10392825" target="_blank" >RIV/00216208:11110/19:10392825 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/19:00337175 RIV/68407700:21670/19:00337175 RIV/00216208:11120/19:43917768
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=o4m8ksefh6" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=o4m8ksefh6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11604-019-00830-6" target="_blank" >10.1007/s11604-019-00830-6</a>
Alternative languages
Result language
angličtina
Original language name
Ethanol fixation method for heart and lung imaging in micro-CT
Original language description
PURPOSE: The soft tissue imaging in micro-CT remains challenging due to its low intrinsic contrast. The aim of this study was to create a simple staining method omitting the usage of contrast agents for ex vivo soft tissue imaging in micro-CT. MATERIALS AND METHODS: Hearts and lungs from 30 mice were used. Twenty-seven organs were either fixed in 97% or 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 h on a custom-built micro-CT machine and compared to scans of three native samples. RESULTS: Ethanol provided contrast enhancement in all evaluated fixations. Fixation in 97% ethanol resulted in contrast enhancement after 72 h; however, it caused hardening of the samples. Fixation in 50% ethanol provided contrast enhancement after 336 h, with milder hardening, compared to the 97% ethanol fixation, but the visualization of details was worse. The fixation in a series of ascending ethanol concentrations provided the most satisfactory results; all organs were visualized in great detail without tissue damage. CONCLUSIONS: Simple ethanol fixation improves the tissue contrast enhancement in micro-CT. The best results can be obtained with fixation of the soft tissue samples in a series of ascending ethanol concentrations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
<a href="/en/project/EF16_019%2F0000766" target="_blank" >EF16_019/0000766: Engineering applications of microworld physics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Japanese Journal of Radiology
ISSN
1867-1071
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
6
Country of publishing house
JP - JAPAN
Number of pages
11
Pages from-to
500-510
UT code for WoS article
000469834600009
EID of the result in the Scopus database
2-s2.0-85062987205