Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10409294" target="_blank" >RIV/00216208:11110/20:10409294 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/20:10409294 RIV/00216208:11140/20:10409294 RIV/00669806:_____/20:10409294 RIV/00064203:_____/20:10409294 RIV/00023736:_____/20:00012763
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dsgsC3-2n6" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dsgsC3-2n6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/bs.mie.2019.05.034" target="_blank" >10.1016/bs.mie.2019.05.034</a>
Alternative languages
Result language
angličtina
Original language name
Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood
Original language description
Natural killer (NK) cells constitute the predominant innate lymphocyte subset that mediates the anti-viral and anti-tumor immune responses. NK cells use an array of innate receptors to sense their environment and to respond to infections, cellular stress and transformation. The resulting NK cell activation, including cytotoxicity and cytokine production, is a fundamental component of the early immune response. The most recent discoveries in NK cell biology have stimulated the translational research that has led to remarkable results for the treatment of human malignancies. Therefore, the rapid isolation of NK cells from the peripheral blood or tumor microenvironment and the subsequent assessment of cytolytic function are crucial to the study of their potency and NK cell-mediated immunosurveillance. Here, we provide protocols for NK cell isolation and the assessment of NK cell cytotoxicity using flow cytometry. (C) 2020 Elsevier Inc.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30102 - Immunology
Result continuities
Project
<a href="/en/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Enzymology
ISSN
0076-6879
e-ISSN
—
Volume of the periodical
631
Issue of the periodical within the volume
Cellular Methods Pt A
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
277-287
UT code for WoS article
000556272600015
EID of the result in the Scopus database
2-s2.0-85067804218