Sensitive CE-MS method for monitoring of riociguat and desmethylriociguat levels in human serum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10413160" target="_blank" >RIV/00216208:11110/20:10413160 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/20:43920284 RIV/00216208:11310/20:10413160 RIV/00216275:25310/20:39916190 RIV/00064165:_____/20:10413160
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Xim-qvslNn" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Xim-qvslNn</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202000135" target="_blank" >10.1002/elps.202000135</a>
Alternative languages
Result language
angličtina
Original language name
Sensitive CE-MS method for monitoring of riociguat and desmethylriociguat levels in human serum
Original language description
Riociguat is novel antihypertensive drug for treatment of pulmonary hypertension. As such, it is still being tested in many clinical and pharmacokinetic trials. Existing methods that determine serum riociguat and desmethylriociguat (DMR) are based solely on liquid chromatography with mass spectrometry. Therefore, we present a novel capillary electrophoresis with mass spectrometry method (CE-MS) for their determination in human serum as alternative method for ongoing trials. Complete resolution of both analytes was achieved by means of pH optimization of ammonium formate background electrolytes that are fully compatible with ESI/MS detection. Simple liquid-liquid extraction was used as sample pretreatment. The calibration dependence of the method was linear (in the range of 10-1000 ng/mL), with adequate accuracy (90.1-114.9%) and precision (13.4%). LOD and LOQ were arbitrarily set at 10 ng/mL for both analytes. Clinical applicability was validated using serum samples from patients treated with riociguat in pharmacokinetic study and the results corresponded with reference HPLC-MS/MS values. Capillary electrophoresis proved to be sensitive and selective tool for the analysis of riociguat and DMR.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
41
Issue of the periodical within the volume
18-19
Country of publishing house
DE - GERMANY
Number of pages
4
Pages from-to
1564-1567
UT code for WoS article
000554033200001
EID of the result in the Scopus database
2-s2.0-85089146202