Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43918840" target="_blank" >RIV/60461373:22330/19:43918840 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0048969719308447?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0048969719308447?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.scitotenv.2019.02.336" target="_blank" >10.1016/j.scitotenv.2019.02.336</a>
Alternative languages
Result language
angličtina
Original language name
Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies
Original language description
Within this study, a new analytical strategy was developed and validated for the simultaneous determination of 78 organohalogenated contaminants in human blood serum, namely 40 flame retardants (FRs) including 7 “novel” brominated and chlorinated FRs (novel FRs), 19 perfluoroalkylated substances (PFASs), 11 organochlorine pesticides (OCPs) and 8 polychlorinated biphenyls (PCBs). The integral sample preparation procedure was implemented for the isolation of non-polar compounds, based on three-step solvent extraction using a mixture of n-hexane:diethylether (9:1, v/v), followed by purification using a solid-phase extraction (SPE) on a Florisil® column. For isolation of more polar and lipophobic analytes, the remaining fraction from the first extraction step was further processed, using a modified QuEChERS method. Depending on the polarity and volatility of target compounds, either gas chromatography coupled to (tandem) mass spectrometry (GC–MS/(MS)), or ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UHPLC-MS/MS), was employed for their identification/quantification. Within the subsequent pilot study, the new validated procedure was successfully applied to the monitoring of organohalogenated contaminants in 38 samples of human blood serum obtained from Prague, Czech Republic. From 78 targeted analytes, 10 PFASs, 10 OCPs, 8 PCBs and 6 BFRs were detected in serum at concentrations above method quantification limits (MQLs). In the serum samples, the amounts of determined PFASs were in the range < 0.01–8.97 ng mL −1 (mean 0.631 ng mL −1 ), OCPs and PCBs ranged from <0.1–1626 ng g −1 lw (mean 40.0 ng g −1 lw) and < 0.1–481 ng g −1 lw (mean 63.3 ng g −1 lw), respectively. © 2019 Elsevier B.V.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LO1601" target="_blank" >LO1601: Prague University Analytical Centre II and III - NPU 2015-2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science of the Total Environment
ISSN
0048-9697
e-ISSN
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Volume of the periodical
667
Issue of the periodical within the volume
JUN 1 2019
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
701-709
UT code for WoS article
000461676600072
EID of the result in the Scopus database
2-s2.0-85062399100