A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10414639" target="_blank" >RIV/00216208:11110/20:10414639 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/20:00117827 RIV/00064165:_____/20:10414639
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qiWh~yLIYZ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qiWh~yLIYZ</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-020-61280-z" target="_blank" >10.1038/s41598-020-61280-z</a>
Alternative languages
Result language
angličtina
Original language name
A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin
Original language description
Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 +/- 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 +/- 2.2 vs. 10.3 +/- 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30209 - Paediatrics
Result continuities
Project
<a href="/en/project/NV18-07-00342" target="_blank" >NV18-07-00342: Studies of the bilirubin degradation products formed during phototherapy of neonatal jaundice</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
—
Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
4411
UT code for WoS article
000563374600009
EID of the result in the Scopus database
2-s2.0-85081602730