DNA methylation signatures to predict the cervicovaginal microbiome status
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10418818" target="_blank" >RIV/00216208:11110/20:10418818 - isvavai.cz</a>
Alternative codes found
RIV/00064211:_____/20:W0000003 RIV/00064165:_____/20:10418818
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y8HD4rGCXW" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y8HD4rGCXW</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13148-020-00966-7" target="_blank" >10.1186/s13148-020-00966-7</a>
Alternative languages
Result language
angličtina
Original language name
DNA methylation signatures to predict the cervicovaginal microbiome status
Original language description
Background: The composition of the microbiome plays an important role in human health and disease. Whether there is a direct association between the cervicovaginal microbiome and the host's epigenome is largely unexplored. Results: Here we analyzed a total of 448 cervicovaginal smear samples and studied both the DNA methylome of the host and the microbiome using the Illumina EPIC array and next-generation sequencing, respectively. We found that those CpGs that are hypo-methylated in samples with non-lactobacilli (O-type) dominating communities are strongly associated with gastrointestinal differentiation and that a signature consisting of 819 CpGs was able to discriminate lactobacilli-dominating (L-type) from O-type samples with an area under the receiver operator characteristic curve (AUC) of 0.84 (95% CI = 0.77-0.90) in an independent validation set. The performance found in samples with more than 50% epithelial cells was further improved (AUC 0.87) and in women younger than 50 years of age was even higher (AUC 0.91). In a subset of 96 women, the buccal but not the blood cell DNA showed the same trend as the cervicovaginal samples in discriminating women with L- from O-type cervicovaginal communities. Conclusions: These findings strongly support the view that the epithelial epigenome plays an essential role in hosting specific microbial communities.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30214 - Obstetrics and gynaecology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Epigenetics
ISSN
1868-7075
e-ISSN
—
Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
180
UT code for WoS article
000595685700001
EID of the result in the Scopus database
2-s2.0-85096435599