Prion Strains Differ in Susceptibility to Photodynamic Oxidation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F22%3A10442549" target="_blank" >RIV/00216208:11110/22:10442549 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dLdCLnyjob" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dLdCLnyjob</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules27030611" target="_blank" >10.3390/molecules27030611</a>

Result language

angličtina

Original language name

Prion Strains Differ in Susceptibility to Photodynamic Oxidation

Original language description

Prion disorders, or transmissible spongiform encephalophaties (TSE), are fatal neurodegenerative diseases affecting mammals. Prion-infectious particles comprise of misfolded pathological prion proteins (PrPTSE). Different TSEs are associated with distinct PrPTSE folds called prion strains. The high resistance of prions to conventional sterilization increases the risk of prion transmission in medical, veterinary and food industry practices. Recently, we have demonstrated the ability of disulfonated hydroxyaluminum phthalocyanine to photodynamically inactivate mouse RML prions by generated singlet oxygen. Herein, we studied the efficiency of three phthalocyanine derivatives in photodynamic treatment of seven mouse adapted prion strains originating from sheep, human, and cow species. We report the different susceptibilities of the strains to photodynamic oxidative elimination of PrPTSE epitopes: RML, A139, Fu-1 &gt; mBSE, mvCJD &gt; ME7, 22L. The efficiency of the phthalocyanine derivatives in the epitope elimination also differed (AlPcOH(SO3)(2) &gt; ZnPc(SO3)(1-3) &gt; SiPc(OH)(2)(SO3)(1-3)) and was not correlated to the yields of generated singlet oxygen. Our data suggest that the structural properties of both the phthalocyanine and the PrPTSE strain may affect the effectiveness of the photodynamic prion inactivation. Our finding provides a new option for the discrimination of prion strains and highlights the necessity of utilizing range of prion strains when validating the photodynamic prion decontamination procedures.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30303 - Infectious Diseases

Project

<a href="/en/project/NV18-04-00179" target="_blank" >NV18-04-00179: New possibilities of intravital diagnostics of prion diseases from peripheral tissues and cerebrospinal fluid.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecules

ISSN

1420-3049

e-ISSN

—

Volume of the periodical

27

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

611

UT code for WoS article

000759547100001

EID of the result in the Scopus database

2-s2.0-85123016063