Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F19%3A10396769" target="_blank" >RIV/00216208:11110/19:10396769 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10396769 RIV/49777513:23220/19:43955399
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Bo5hM53Rwc" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Bo5hM53Rwc</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jbio.201800430" target="_blank" >10.1002/jbio.201800430</a>

Result language
angličtina
Original language name
Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen
Original language description
Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrP (c)) into its misfolded infectious form (PrPTSE). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3)(2)) utilizing two custom-built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O-2((1)Delta(g))) is the only species significantly photogenerated by AlPcOH(SO3)(2), and it is responsible for the PDI of prions. More intensive light conditions show not only higher O-2((1)Delta(g)) production but also decreases in AlPcOH(SO3)(2) photostability. Our findings suggest that PDI by AlPcOH(SO3)(2)-generated O-2((1)Delta(g)) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30102 - Immunology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)