Monitoring of DNA structural changes after incorporation of the phenylpyrazole insecticide fipronil
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10482953" target="_blank" >RIV/00216208:11110/24:10482953 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=n40SKkEUJn" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=n40SKkEUJn</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.abb.2024.110001" target="_blank" >10.1016/j.abb.2024.110001</a>
Alternative languages
Result language
angličtina
Original language name
Monitoring of DNA structural changes after incorporation of the phenylpyrazole insecticide fipronil
Original language description
The use of insecticides presents a risk to the environment because they can accumulate in the water, soil, air, and organisms, endangering human and animal health. It is therefore essential to investigate the effects of different groups of insecticides on individual biomacromolecules such as DNA. We studied fipronil, which belongs to the group of phenylpyrazole insecticides. The interaction of fipronil with calf thymus DNA was investigated using spectroscopic methods (absorption and fluorescence spectroscopy) complemented with infrared spectroscopy and viscosity measurement. Fluorescence emission spectroscopy showed the formation of a fipronil/DNA complex with a combined static and dynamic type of quenching. The binding constant was 4.15 x 103 L/mol. Viscosity changes were recorded to confirm/disconfirm the intercalation mode of interaction. A slight change in DNA viscosity in the presence of fipronil was observed. The phenylpyrazole insecticide does not cause significant conformational changes in DNA structure or increase of its chain length. We hypothesize that fipronil is incorporated into the minor groove of the DNA macromolecule via hydrogen interactions as indicated by FT-IR and CD measurements.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics
Result continuities
Project
—
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Biochemistry and Biophysics
ISSN
0003-9861
e-ISSN
1096-0384
Volume of the periodical
756
Issue of the periodical within the volume
June
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
110001
UT code for WoS article
001235646700001
EID of the result in the Scopus database
2-s2.0-85191307468