SMARCA5-mediated chromatin remodeling is required for germinal center formation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10485802" target="_blank" >RIV/00216208:11110/24:10485802 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=pQ4b9Ax675" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=pQ4b9Ax675</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1084/jem.20240433" target="_blank" >10.1084/jem.20240433</a>
Alternative languages
Result language
angličtina
Original language name
SMARCA5-mediated chromatin remodeling is required for germinal center formation
Original language description
B cells undergo extensive genomic reorganization during their proliferation and differentiation into germinal center and antibody-secreting cells. Stoler-Barak et al. demonstrate that SMARCA5, a component of the ISWI-complex, plays a critical role in gene accessibility and expression, facilitating the antibody-mediated immune response. The establishment of long-lasting immunity against pathogens is facilitated by the germinal center (GC) reaction, during which B cells increase their antibody affinity and differentiate into antibody-secreting cells (ASC) and memory cells. These events involve modifications in chromatin packaging that orchestrate the profound restructuring of gene expression networks that determine cell fate. While several chromatin remodelers were implicated in lymphocyte functions, less is known about SMARCA5. Here, using ribosomal pull-down for analyzing translated genes in GC B cells, coupled with functional experiments in mice, we identified SMARCA5 as a key chromatin remodeler in B cells. While the naive B cell compartment remained unaffected following conditional depletion of Smarca5, effective proliferation during B cell activation, immunoglobulin class switching, and as a result GC formation and ASC differentiation were impaired. Single-cell multiomic sequencing analyses revealed that SMARCA5 is crucial for facilitating the transcriptional modifications and genomic accessibility of genes that support B cell activation and differentiation. These findings offer novel insights into the functions of SMARCA5, which can be targeted in various human pathologies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Medicine
ISSN
0022-1007
e-ISSN
1540-9538
Volume of the periodical
221
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
21
Pages from-to
e20240433
UT code for WoS article
001316111900001
EID of the result in the Scopus database
2-s2.0-85204759274