Long-term exposure to moderate cold reduces incidence of reperfusion tachyarrhythmias in rats
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F25%3A10510832" target="_blank" >RIV/00216208:11110/25:10510832 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10510832
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=wGFHv.yzga" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=wGFHv.yzga</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1152/japplphysiol.00509.2025" target="_blank" >10.1152/japplphysiol.00509.2025</a>
Alternative languages
Result language
angličtina
Original language name
Long-term exposure to moderate cold reduces incidence of reperfusion tachyarrhythmias in rats
Original language description
Recently, moderate cold acclimation (9 °C; MCA) was found to exert cardioprotective effects by increasing resistance to ischemia-reperfusion (IR) injury and mitochondrial calcium overload in rats, but the effect of MCA on the incidence of IR arrhythmias is not known. The aim of this study was to determine whether MCA can induce an antiarrhythmic effect and, if so, to elucidate a possible mechanism. Adult male Wistar rats were acclimated (9 °C) for short (1-3-10 days) and long-term (5 wk; CA) periods, followed by a 2-wk recovery period (24 °C; CAR). The number of premature ventricular complexes (PVCs) and their duration after IR were determined. Left ventricular myocardium was analysed by western blotting, TLC & GC chromatography and immunofluorescence microscopy. We revealed that total reperfusion PVCs and tachyarrhythmia duration decreased even after CA, and accordingly, the antiarrhythmic n-3 PUFAs increased in cardiac membrane phospholipids, the n-6/n-3 PUFA ratio decreased. CA increased the distribution of connexin 43 (Cx43) in favor of end-to-end junctions and the expression of uncoupling protein 3 (UCP3) in mitochondria. These beneficial effects were lost after 2-wk recovery period CAR. Interestingly, the mitochondrial antioxidants superoxide dismutase (SOD2) and thioredoxin reductase (TRXRD2) were strongly upregulated exclusively after 1 day of cold exposure, whereas cytosolic TRXRD1 was downregulated. In conclusion, long-term MCA (5-wk) reduces the incidence of reperfusion arrhythmias, increases the proportion of antiarrhythmic n-3PUFAs in cardiomyocyte membranes, and has a positive effect on Cx43 distribution. By increasing the amount of UCP3 in mitochondria it may reduce the likelihood of free radical formation in mitochondria during reperfusion.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Physiology
ISSN
8750-7587
e-ISSN
1522-1601
Volume of the periodical
139
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
1156-1168
UT code for WoS article
001620743400003
EID of the result in the Scopus database
2-s2.0-105019386109