CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F05%3A00003842" target="_blank" >RIV/00216208:11120/05:00003842 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1634/stemcells.2004-0291" target="_blank" >http://dx.doi.org/10.1634/stemcells.2004-0291</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1634/stemcells.2004-0291" target="_blank" >10.1634/stemcells.2004-0291</a>
Alternative languages
Result language
angličtina
Original language name
CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
Original language description
Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, phenotype, and distribution. Single-cell suspensions from normal mouse corneas were also prepared and analyzed by flow cytometry. After short-term culture of corneal stromal explants, nonadherent cells were harvested and cytospins were prepared and stained for different markers. Combined staining for F-actin and leukocyte differentiation markers clearly showed that the corneal stroma contains a population of CD45(+) resident bone marrow-derived cells, whereas most cells were CD45-F-actin(+) keratocytes. A significant proportion (two thirds) of CD45(+) cells in the normal corneal stroma expressed CD34(+), whereas no CD45(-) c
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem Cells
ISSN
1066-5099
e-ISSN
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Volume of the periodical
23
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
507-515
UT code for WoS article
000228161800009
EID of the result in the Scopus database
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