Acute exposure to long-chain fatty acid impairs alpha2-adrenergic mediated antilipolysis in human adipose tissue
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F07%3A00000285" target="_blank" >RIV/00216208:11120/07:00000285 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Acute exposure to long-chain fatty acid impairs alpha2-adrenergic mediated antilipolysis in human adipose tissue
Original language description
The acute in vitro and in vivo effects of long-chain fatty acids (LCFAs) on the regulation of adrenergic lipolysis were investigated in human adipose tissue. The effect of a 2 h incubation, without or with LCFA (200 mumol/l), on basal and hormonally induced lipolysis was tested in vitro on isolated fat cells. The lipolytic response to epinephrine was enhanced by suppression of the antilipolytic alpha(2)-adrenergic effect. Then, healthy lean and obese male subjects performed a 45 min exercise bout at 50%of their heart rate reserve either after an overnight fast or 3 h after a high-fat meal (HFM: 95% fat, 5% carbohydrates). Subcutaneous adipose tissue lipolysis was measured by microdialysis in the presence or absence of an alpha-antagonist (phentolamine). In vivo, a HFM increased plasma levels of nonesterified fatty acids in lean and obese subjects. In both groups, the HFM did not alter hormonal responses to exercise. Under fasting conditions, the alpha(2)-adrenergic antilipolytic effec
Czech name
Vliv akutní expozice mastným kyselinám s dlouhým řeztězcem na alfa2 adrenergní antilipolytický efekt v lidské tukové tkáni
Czech description
Cílem studie bylo sledovat vliv akutní expozice mastným kyselinám na regulaci adrenergní lipolýzy in vivi a in vitro. Lipolytický efekt byl zvýšem po 2 hod pre-inkubaci s mastnými kyselinami, zejmína díky potlačení alpha(2)-adrenergního anti-lipolytického efektu in vitro. In vivo byla seldována lipolýza během fyzické zátěže u doborovlníků na lačno nebo po požití vysokotučného pokrmu. Na lačno byl alpha(2)-adrenergní anti-lipolytický efekt patrný o obézních jedinců a po příjmu vysokotučného pokrmu byl zcela elimninován, což demonstruje účast mastných kyselin an regulaci lipolýzy
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Lipid Research
ISSN
0022-2275
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
2236-2246
UT code for WoS article
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EID of the result in the Scopus database
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