A Determination of Submicromolar Concentrations of Glycine in Periaqueductal Gray Matter Microdialyzates Using Capillary Zone Electrophoresis with Contactless Conductivity Detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F09%3A00001676" target="_blank" >RIV/00216208:11120/09:00001676 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A Determination of Submicromolar Concentrations of Glycine in Periaqueductal Gray Matter Microdialyzates Using Capillary Zone Electrophoresis with Contactless Conductivity Detection

Original language description

Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 ?M by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F07%2F0896" target="_blank" >GA203/07/0896: Fast screening of biologically active low-molecular weight compounds in biological materials by microfluidic techniques</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

30

Issue of the periodical within the volume

19

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

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UT code for WoS article

000271154100019

EID of the result in the Scopus database

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