Large-volume sample stacking for in vivo monitoring of trace levels of gamma-aminobutyric acid, glycine and glutamate in microdialysates of periaqueductal gray matter by capillary electrophoresis with contactless conductivity detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10144149" target="_blank" >RIV/00216208:11310/13:10144149 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/13:43907478
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2013.06.019" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2013.06.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2013.06.019" target="_blank" >10.1016/j.chroma.2013.06.019</a>

Result language
angličtina
Original language name
Large-volume sample stacking for in vivo monitoring of trace levels of gamma-aminobutyric acid, glycine and glutamate in microdialysates of periaqueductal gray matter by capillary electrophoresis with contactless conductivity detection
Original language description
A new variant of large-volume sample stacking injection (LVSS) was used in the capillary electrophoresis with capacitively coupled contactless conductivity detection (CE/(CD)-D-4) determination of the neurotransmitters gamma-aminobutyric acid (GABA), glycine (Gly) and glutamate (Glu) in microdialysates of periaqueductal gray matter (PAG). The separation capillary was filled to 98% from the injection side with a sample of microdialysate in acetonitrile. Simultaneously with turning on the separation voltage, the sample zone was forced out by the background electrolyte by increasing the pressure in the terminal capillary outlet vessel. As a consequence of the stacking effect, the analyte was concentrated from the large sample volume into a narrow zone atthe sample/background electrolyte boundary close to the injection end of the capillary. Under these conditions, LOD values of 9, 10 and 15 nM were determined in the model samples for GABA, Gly and Glu, respectively; RSD equalled 0.5% for
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—

Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)