Interlaboratory variability of CD34+stem cell enumeration. A pilot study to national external quality assessment within the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F10%3A00002904" target="_blank" >RIV/00216208:11120/10:00002904 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/10:6329 RIV/00179906:_____/10:10084222 RIV/00064203:_____/10:6329 RIV/00023736:_____/10:00008922 and 2 more
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Interlaboratory variability of CD34+stem cell enumeration. A pilot study to national external quality assessment within the Czech Republic
Original language description
We present the results of a pilot study concerning the interlaboratory variability of CD34+ enumeration. Three surveys, each including a set of samples, were sent to participating Czech flow cytometry laboratories. The efficacy of this exercise was determined by the reduction in interlaboratory variation and the influence of method used on assay outcome. The variability in results of CD34+ enumeration declined with time. The mean coefficient of variation (CV) of measurement among laboratories dropped, from 58% in first survey to 32% in last survey. All tested variables (gating strategy, platform methodology, sample preparation) affected the variability of the assay. Sample preparation method was associated with a significant bias of absolute CD34+ cellcounts. Initially, the outcome of the measurement was also affected by the participating laboratory (identified by a unique laboratory number; ULN). However, laboratories with poorer performance modified their protocols during the study,
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NR9268" target="_blank" >NR9268: The role of adhesion molecules and cytokines in hematopoirtic stem cell mobilization in healthy donors</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Laboratory Hematology
ISSN
1751-5521
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
6, Part
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
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UT code for WoS article
000283595500006
EID of the result in the Scopus database
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