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Very Fast Electrophoretic Separation of Neurotransmitters on Commercial Instruments Using a Coupled Capillary

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F12%3A43908250" target="_blank" >RIV/00216208:11120/12:43908250 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.bestservis.eu/upload/file/Sbornik_metody12.pdf" target="_blank" >http://www.bestservis.eu/upload/file/Sbornik_metody12.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Very Fast Electrophoretic Separation of Neurotransmitters on Commercial Instruments Using a Coupled Capillary

  • Original language description

    A capillary formed by connecting of two capillaries with different inner diameters (25 and 100 ?m) was tested for electrophoretic separation at high electric field intensities. The laboratory made coupled capillary was directly placed into the cassette of Agilent CE apparatus and was tested for separation of dopamine, noradrenaline and adrenaline in a background electrolyte of 20 mM citric acid/NaOH, pH 3.2. An intensity of 2.7 kV cm-1 was attained in the separation part of the capillary, which is 2.9 times more than maximum intensity value attainable in the common capillary with the shortest possible length at maximum voltage +30 kV. At these high electric field intensities, the migration times were 12.3 s (dopamine), 12.8 s (noradrenaline), 13.3 s (adrenaline); and the attained separation efficiencies were between 2350 and 2760 plates s-1.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    CECE 2012 - 9th International Interdisciplinary Meeting on Bioanalysis

  • ISBN

    978-80-904959-1-3

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    213-216

  • Publisher name

    Institute of Analytical Chemistry AV CR

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 1, 2012

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000329635000062