Very fast electrophoretic separation on commercial instruments using a combination of two capillaries with different internal diameters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43906901" target="_blank" >RIV/00216208:11120/13:43906901 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/13:10133792

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201200524" target="_blank" >http://dx.doi.org/10.1002/elps.201200524</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201200524" target="_blank" >10.1002/elps.201200524</a>

Result language

angličtina

Original language name

Very fast electrophoretic separation on commercial instruments using a combination of two capillaries with different internal diameters

Original language description

A capillary formed by connecting a 9.7 cm long separation capillary with id 25 ?m with an auxiliary 22.9 cm long capillary with id 100 ?m (coupled capillary) was tested for electrophoretic separation at high electric field intensities. The coupled capillary was placed in the cassette of a standard electrophoresis apparatus. It was used in the short-end injection mode for separation of a mixture of dopamine, noradrenaline and adrenaline in a background electrolyte of 20 mM citric acid/NaOH, pH 3.2. An intensity of 2.7 kV cm(-1) was attained in the separation part of the capillary at a separation voltage of 30 kV, which is 2.9 times more than maximum intensity value attainable in a capillary with the same length with uniform id. At these high electric field intensities, the migration times of the tested neurotransmitters had values of 12.3 to 13.3 s and the attained separation efficiency was between 2350 and 2760 plates s(-1) . It is thus demonstrated that an effective separation instrum

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

34

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

552-556

UT code for WoS article

000315169800011

EID of the result in the Scopus database

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