Pepsin Digest of Wheat Gliadin Fraction Increases Production of IL-1 beta via TLR4/MyD88/TRIF/MAPK/NF-kappa B Signaling Pathway and an NLRP3 Inflammasome Activation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43907500" target="_blank" >RIV/00216208:11120/13:43907500 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/13:00399914

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Pepsin Digest of Wheat Gliadin Fraction Increases Production of IL-1 beta via TLR4/MyD88/TRIF/MAPK/NF-kappa B Signaling Pathway and an NLRP3 Inflammasome Activation

Original language description

Celiac disease (CD) is a gluten-responsive, chronic inflammatory enteropathy. IL-1 cytokine family members IL-1 beta and IL-18 have been associated with the inflammatory conditions in CD patients. However, the mechanisms of IL-1 molecule activation in CDhave not yet been elucidated. We show in this study that peripheral blood mononuclear cells (PBMC) and monocytes from celiac patients responded to pepsin digest of wheat gliadin fraction (PDWGF) by a robust secretion of IL-1 beta and IL-1 alpha and a slightly elevated production of IL-18. The analysis of the upstream mechanisms underlying PDWGF-induced IL-1 beta production in celiac PBMC show that PDWGF-induced de novo pro-IL-1 beta synthesis, followed by a caspase-1 dependent processing and the secretion of mature IL-1 beta. This was promoted by K+ efflux and oxidative stress, and was independent of P2X7 receptor signaling. The PDWGF-induced IL-1 beta release was dependent on Nod-like receptor family containing pyrin domain 3 (NLRP3)

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

"e62426; 1"-11

UT code for WoS article

000321662800044

EID of the result in the Scopus database

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