Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43907514" target="_blank" >RIV/00216208:11120/13:43907514 - isvavai.cz</a>

Alternative codes found

RIV/00023001:_____/13:00058736

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201300401" target="_blank" >http://dx.doi.org/10.1002/jssc.201300401</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300401" target="_blank" >10.1002/jssc.201300401</a>

Result language

angličtina

Original language name

Determination of midazolam in rabbit plasma by GC and LC following nasal and ocular administration

Original language description

GC with nitrogen phosphorus detection and HPLC with UV detection were used to determine midazolam levels in rabbit plasma following ocular and nasal administration. For GC with nitrogen phosphorus detection, the analyte was extracted from the plasma using a three-step liquid?liquid extraction including extraction with an isopropanol/butyl chloride mixture in an alkaline solution, followed by extractions with 1 M HCl and finally with an alkaline solution of butyl chloride. The recovery of midazolam was very dependent on the sample alkalization time prior to the final extraction. The procedure increased the recovery of midazolam up to 99.6%. Improved sample preparation led to a significant increase in the sensitivity of the determination by GC with nitrogen phosphorus detection. The achieved detection limit was 0.34 ng/ml, which is ten times lower than that obtained using HPLC with UV detection. The small plasma volume was another advantage of the GC with nitrogen phosphorus detection me

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/NT11284" target="_blank" >NT11284: Non-traditional application of anaesthetic drugs in disaster medicine</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

20

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

3366-3371

UT code for WoS article

000325482500009

EID of the result in the Scopus database

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