Very fast electrophoretic determination of creatinine and uric acid in human urine using a combination of two capillaries with different internal diameters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F14%3A43907632" target="_blank" >RIV/00216208:11120/14:43907632 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201300293" target="_blank" >http://dx.doi.org/10.1002/elps.201300293</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201300293" target="_blank" >10.1002/elps.201300293</a>

Result language

angličtina

Original language name

Very fast electrophoretic determination of creatinine and uric acid in human urine using a combination of two capillaries with different internal diameters

Original language description

A capillary system formed by combining 25 ?m and 100 ?m id capillaries was used in the short-end injection mode to determine creatinine and uric acid in human urine. The separation was performed at an electric field intensity of 2.3 kV/cm. Creatinine wasdetermined in a background electrolyte with a composition of 20 mM citric acid/NaOH (pH 3.0) and uric acid was determined in 20 mM MES/NaOH (pH 6.0). Under these conditions, migration times of 12.2 s for creatinine and 8.6 s for uric acid were achieved.The LOD value is 2.4 mg/L for creatinine and 0.9 mg/L for uric acid; the RSD for the migration time varies in the range 0.7-1.1% (intra day) to 1.0-7.5% (inter day); RSDs for the peak areas equalled 3.4-4.0% (intra day) and 4.3-4.7% (inter day). The determined creatinine values in 7 urine samples vary in the range 221-1394 mg/L for creatinine and 87-615 mg/L for uric acid. T-test did not reveal any statistically significant difference between the developed CE methodologies and reference

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP206%2F11%2F0707" target="_blank" >GAP206/11/0707: Voltammetric assessment of new ionic liquids for their applications in capillary electrophoresis</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

956-961

UT code for WoS article

000333644600004

EID of the result in the Scopus database

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