Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F14%3A43908436" target="_blank" >RIV/00216208:11120/14:43908436 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.016" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.04.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.016" target="_blank" >10.1016/j.chroma.2014.04.016</a>

Result language
angličtina
Original language name
Large Volume Sample Stacking for Rapid and Sensitive Determination of Antidiabetic Drug Metformin in Human Urine and Serum by Capillary Electrophoresis with Contactless Conductivity Detection
Original language description
Two CE methods with contactless conductivity detection have been developed for determining the oral antidiabetic drug metformin in human urine and blood. The determination of metformin is performed on a separation capillary with an effective length of 14cm, using a maximum voltage of 30 kV and with a small injection of 50-fold diluted urine into the capillary. Under these conditions, the migration time of metformin is 35 s and the LOD is 0.3 ?M. Large-volume sample stacking was used to determine low metformin levels in serum. The injection of a sample of serum deproteinized with acetonitrile was 10 times greater compared to the injected amount of urine. This enabled reduction of the LOD to 0.03 ?M and the metformin migration time equalled 86 s. The undesirable solvent from sample zone was forced out of the capillary to ensure rapidity and good repeatability of the determination. The RSD values for the migration time are 0.1 % for urine and 0.7 % for serum; RSD for the peak areas equal
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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