The protective effect of pyrimidine nucleosides on human HaCaT keratinocytes treated with 5-FU

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909503" target="_blank" >RIV/00216208:11120/15:43909503 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/15:10294985 RIV/00064165:_____/15:10294985 RIV/00216305:26620/15:PU125849

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The protective effect of pyrimidine nucleosides on human HaCaT keratinocytes treated with 5-FU

Original language description

Background: Therapy with 5-fluorouracil (5-FU) and capecitabine is often complicated by skin toxicity (hand-foot syndrome, HFS). Topical application of uridine ointment is beneficial for alleviating HFS and other pyrimidine nucleosides have been described as 5-FU toxicity modulators. We tested pyrimidine nucleosides and their combinations to find the best combination for topical therapy of HFS. Materials and methods: Cellular viability was measured by the real-time cell analyser and methyl thiazol tetrazolium (MTT) assay in order to evaluate the effect of pyrimidine nucleosides on HaCaT keratinocytes treated with 5-FU. The results were confirmed by evaluation of the cellular colonization by microphotography. Results: Cytidine and uridine protected keratinocytes to the same extent. Thymidine enhanced the protective effect when added to cytidine or uridine. Deoxycytidine did not have any protective effect. Conclusion: Our findings support the rationale for using uridine or cytidine in combination with thymidine in ointment for HFS treatment.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Anticancer Research

ISSN

0250-7005

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

3

Country of publishing house

GR - GREECE

Number of pages

8

Pages from-to

1303-1310

UT code for WoS article

000352462900011

EID of the result in the Scopus database

2-s2.0-84924995371