The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43910347" target="_blank" >RIV/00216208:11120/15:43910347 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/15:10321964 RIV/61388971:_____/15:00462022

Result on the web

<a href="http://dx.doi.org/10.1515/cmble-2015-0054" target="_blank" >http://dx.doi.org/10.1515/cmble-2015-0054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/cmble-2015-0054" target="_blank" >10.1515/cmble-2015-0054</a>

Result language

angličtina

Original language name

The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes

Original language description

Experiments using cultured primary cells or cell lines are a routine in vitro approach used across multiple biological disciplines, However, the structural and functional influences of various cultureware materials on cultured cells is not clearly understood. Surface treatments of cultureware have proven to have profound effects on cell viability and proliferation. In this study, we investigated the impact of polystyrene and fluorocarbon cultureware dishes on the proteomic profile of differentiated 3T3-L1 preadipocytes. After expansion and differentiation of cells on appropriate cultureware dishes, cell lysates were separated using two-dimensional gel electrophoresis and proteins were visualized with Coomassie blue staining. Spots with the highest differential expression between the two culture conditions were subsequently analyzed using matrix-assisted laser desorption/ionization mass spectrometry and the identified proteins were subjected to pathway analysis. We observed that 43% of all spots were differentially expressed depending on the cultureware. Pathway analysis revealed that glucose metabolism, mitochondrial structure and cell differentiation, represented by 14-3-3 protein-mediated signaling and the mitochondrial inner membrane organizing system (MINOS), were significantly affected by cultureware material. These results indicate that cultureware material can have a profound effect on key adipocyte functional pathways. These effects modifications of the cells should be reflected in the design of in vitro experiments and interpretation of their results.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/GA13-27735S" target="_blank" >GA13-27735S: Modifications in Adipose Tissue Metabolism Leading to Diabetes in Sleep Apnea Syndrome (Study SAMET - Sleep Apnea and Adipose Tissue Metabolism)</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular &amp; Molecular Biology Letters

ISSN

1425-8153

e-ISSN

—

Volume of the periodical

20

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

919-936

UT code for WoS article

000372089200013

EID of the result in the Scopus database

2-s2.0-84962620272