Protein profiling of SH-SY5Y neuroblastoma cells: The effect of rhein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00507834" target="_blank" >RIV/67985823:_____/19:00507834 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10397684 RIV/00159816:_____/19:00072495
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs12038-019-9908-0" target="_blank" >https://link.springer.com/article/10.1007%2Fs12038-019-9908-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12038-019-9908-0" target="_blank" >10.1007/s12038-019-9908-0</a>
Alternative languages
Result language
angličtina
Original language name
Protein profiling of SH-SY5Y neuroblastoma cells: The effect of rhein
Original language description
4,5-Dihydroxyanthraquinone-2-carboxylic acid (Rhein) has been shown to have various physiological and pharmacological properties including anticancer activity and modulatory effects on bioenergetics. In this study, we explored the impact of rhein on protein profiling of undifferentiated (UC) and differentiated (DC) SH-SY5Y cells. Besides that, the cellular morphology and expression of differentiation markers were investigated to determine the effect of rhein on retinoic acid-induced neuronal cell differentiation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry we evaluated the changes in the proteome of both UC and DC SH-SY5Y cells after 24 h treatment with rhein. Validation of selected differentially expressed proteins and the assessment of neuronal differentiation markers were performed by western blotting. Proteomic analysis revealed significant changes in the abundance of 15 proteins linked to specific cellular processes such as cytoskeleton structure and regulation, mitochondrial function, energy metabolism, protein synthesis and neuronal plasticity. We also observed that the addition of rhein to the cultured cells during differentiation resulted in a significantly reduced neurite outgrowth and decreased expression of neuronal markers. These results indicate that rhein may strongly interfere with the differentiation process of SH-SY5Y neuroblastoma cells and is capable of inducing marked proteomic changes in these cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biosciences
ISSN
0250-5991
e-ISSN
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Volume of the periodical
44
Issue of the periodical within the volume
4
Country of publishing house
IN - INDIA
Number of pages
16
Pages from-to
UNSP88
UT code for WoS article
000478891800005
EID of the result in the Scopus database
2-s2.0-85071982312