Detection and cultivation of circulating tumor cells in gastric cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F16%3A43909623" target="_blank" >RIV/00216208:11120/16:43909623 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10327681 RIV/00064173:_____/16:N0000009 RIV/00064203:_____/16:10327681

Result on the web

<a href="http://dx.doi.org/10.1007/s10616-015-9866-9" target="_blank" >http://dx.doi.org/10.1007/s10616-015-9866-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10616-015-9866-9" target="_blank" >10.1007/s10616-015-9866-9</a>

Result language

angličtina

Original language name

Detection and cultivation of circulating tumor cells in gastric cancer

Original language description

Circulating tumor cells (CTCs) are important targets for treatment and critical surrogate markers when evaluating cancer prognosis and therapeutic response. A sensitive methodology for detecting CTCs in gastric cancer (GC) patients is needed. In this study we demonstrate a device for enrichment and cultivation of CTCs. In total, 22 patients with GC, all candidates for surgery, were enrolled in the study. Peripheral blood samples were collected before surgery, and patients were re-evaluated within operation and divided into two groups: resectable and non-resectable GC. A new size-based separation test for enrichment and cultivation of CTCs was used (MetaCell(R)). In addition to cytomorphological analysis, gene expression of tumor associated genes (Cytokeratin-18, Cytokeratin-19, Cytokeratin-20, Cytokeratin-7, EPCAM, MUC1, HER2, EGFR) and of leukocyte markers (e.g. CD45, CD68) was tested in enriched CTC fractions. CTCs were detected in 59 % of the patients studied (n = 13/22). CTCs were detected in seven patients of the resection group (7/10, 70 %) and six of the non-resectable group (6/12, 50 %). Enrichment of the viable CTCs allowed subsequent successful cultivation in vitro. The cytomorphological characterization of the CTCs was a prerequisite of random gene expression testing in CTC-positive samples. In CTC-positive samples gene expression of cytokeratin 18 and 19 was elevated in comparison to the whole blood gene expression analysis. CTCs were found to be present in both resectable and non-resectable gastric cancer patients. The size-based separation platform for CTCs may be used for in vitro cultivation, as well as in subsequent molecular analysis if desired. The sensitivity of CTC-detection could be enhanced by the combination of cytomorphological and molecular analysis.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/NT14439" target="_blank" >NT14439: Quantification of circulating tumor cells (CTC) in tumors of gastrointestinal tract</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytotechnology

ISSN

0920-9069

e-ISSN

—

Volume of the periodical

68

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

1095-1102

UT code for WoS article

000380265300047

EID of the result in the Scopus database

2-s2.0-84927536672