Isolation, primary culture, morphological and molecular characterization of circulating tumor cells in gynecological cancers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10301827" target="_blank" >RIV/00216208:11110/15:10301827 - isvavai.cz</a>
Alternative codes found
RIV/00064173:_____/15:#0000453 RIV/00064203:_____/15:10301827
Result on the web
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548313/pdf/ajtr0007-1203.pdf" target="_blank" >http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548313/pdf/ajtr0007-1203.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Isolation, primary culture, morphological and molecular characterization of circulating tumor cells in gynecological cancers
Original language description
The focus of the study was to implement a new workflow for circulating tumor cells (CTCs) characterization that would allow the analysis of CTCs on a cytomorphological and molecular level in patients with diagnosed gynecological cancer. Our findings maybe useful in future cancer patient management. The study introduces a size-based enrichment (MetaCell (R)) method for the separation of viable CTCs, followed by CTCs culturing in vitro and gene expression characterization. It is based on the observationof CTCs and DTCs (Disseminated Tumor Cells) in several case studies of ovarian, endometrial and cervical cancer by means of cytomorphology and gene expression profiling. The viability of the enriched CTCs was estimated using vital and lethal fluorescencenuclear staining. This type of staining may be predictive for the success rate of subsequent CTC growth in vitro. To identify CTCs in the enriched CTC fraction, cytomorphological evaluations based on vital fluorescence staining were foll
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT14441" target="_blank" >NT14441: Circulating and disseminated tumor cells in diagnosis and treatment of advanced ovarian cancer.</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
American Journal of Translational Research
ISSN
1943-8141
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1203-1213
UT code for WoS article
000361558000002
EID of the result in the Scopus database
2-s2.0-84939535871