Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43917764" target="_blank" >RIV/00216208:11120/19:43917764 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/978-1-4939-9213-3_2" target="_blank" >https://doi.org/10.1007/978-1-4939-9213-3_2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-9213-3_2" target="_blank" >10.1007/978-1-4939-9213-3_2</a>

Result language
angličtina
Original language name
Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies
Original language description
Capillary electrophoresis (CE) with contactless conductivity detection (C 4 D) represents a strong tool for determining amino acids in clinical samples. This chapter provides detailed instructions for CE/C 4 D determination of the branched-chain amino acids (BCAAs) valine, isoleucine, and leucine in human plasma, which can be readily employed in physiological studies. Baseline separation of all the BCAAs is achieved on a short separation length equal to 18 cm in optimized background electrolyte consisting of 3.2 M acetic acid dissolved in 20% v/v methanol with addition of 1.0% v/v INST-coating solution. The analysis time does not exceed 3 min and the limit of detection is 0.4 μM for all BCAAs. The pretreatment of human plasma is very simple and is based on fourfold plasma dilution by acetonitrile and subsequent filtration. Only 50 μL of plasma is used for the analysis. The high sensitivity of the CE/C 4 D method is achieved by injecting a large volume of sample, combined with application of negative pressure to flush the acetonitrile zone out of the capillary.
Czech name
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Czech description
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Project
<a href="/en/project/GA18-04902S" target="_blank" >GA18-04902S: Instrumentation for continuous on-line electtrophoretic monitoring of metabolic processes in living organisms</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
Clinical Applications of Capillary Electrophoresis: Methods and Protocols
ISBN
978-1-4939-9212-6
Number of pages of the result
10
Pages from-to
15-24
Number of pages of the book
273
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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