Dialysis of one sample drop on-line connected with electrophoresis in short capillary

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43920261" target="_blank" >RIV/00216208:11120/20:43920261 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/20:10413172

Result on the web

<a href="https://doi.org/10.1016/j.talanta.2020.121252" target="_blank" >https://doi.org/10.1016/j.talanta.2020.121252</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2020.121252" target="_blank" >10.1016/j.talanta.2020.121252</a>

Result language

angličtina

Original language name

Dialysis of one sample drop on-line connected with electrophoresis in short capillary

Original language description

An analytical apparatus is described, based on on-line connection of electrophoresis in a short capillary with a dialysis unit enabling dialysis in micro-litre sample volumes into submicro-litre volumes of an acceptor solution in a dialysing fibre. After a defined dialysis time, the dialysate from the dialysing fibre is injected into a separation capillary through an air-assisted flow-gating interface cast from PDMS. In the flow-gating injection space, the exit from the delivery capillary bringing the dialysate is placed directly opposite the entrance into the separation capillary at a distance of 380 μm. In order to enable injection of a very small volume of dialysate, the background electrolyte is forced out of the injection space with air before the injection, so that a drop of dialysate with a volume of about 0.1 μL is formed between the exit from the delivery and the entrance into the separation capillary; the dialysate is injected hydrodynamically from this dialysate drop. Then the injection space is filled with the background electrolyte and the separation is commenced. The basic properties of the apparatus were tested on model mixtures of inorganic cations (K+, Ba2+ and Na+) and organic molecules (creatinine, histidine and arginine). The applicability to real samples was tested on the determination of basic amino acids (histidine, lysine and arginine) in a blood serum sample.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA18-04902S" target="_blank" >GA18-04902S: Instrumentation for continuous on-line electtrophoretic monitoring of metabolic processes in living organisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

—

Volume of the periodical

219

Issue of the periodical within the volume

November

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

"Article 121252"

UT code for WoS article

000568744000002

EID of the result in the Scopus database

2-s2.0-85086641814