Characterization of various geometric arrangements of "Air-assisted" flow gating interfaces for capillary electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F21%3A43920777" target="_blank" >RIV/00216208:11120/21:43920777 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/21:10417623

Result on the web

<a href="https://doi.org/10.1002/elps.202000305" target="_blank" >https://doi.org/10.1002/elps.202000305</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.202000305" target="_blank" >10.1002/elps.202000305</a>

Result language

angličtina

Original language name

Characterization of various geometric arrangements of "Air-assisted" flow gating interfaces for capillary electrophoresis

Original language description

For connecting flow-through analytical methods with capillary electrophoresis, a chip working in the air-assisted flow gating interface regime is cast from poly(dimethylsiloxane). In the injection space, the exit from the delivery capillary is placed close to the entrance to the separation capillary. Prior to injecting the sample into the separation capillary, the background electrolyte is forced out of the injection space by a stream of air. In the empty space, a drop of the sample with a volume of &lt; 100 nL is formed between the exit from the delivery capillary and the entrance into the separation capillary, from which the sample is injected hydrodynamically into the separation capillary. After injection, the injection space is filled with BGE and the separation can be begun. Three geometric variants for the mutual geometric arrangement of the delivery and separation capillaries were tested: the delivery capillary is placed perpendicular to the separation capillary, from either above or below, or the capillaries are placed axially, i.e., directly opposite one another. All of the variants are equivalent from the analytical and separation efficiency viewpoints. The repeatability expressed by RSD is up to 5%. The tested flow gating interface variants are also suitable for continuous and discontinuous sampling at flow rates of the order of units of μL/min. The developed instrument for sequential electrophoretic analysis operates fully automatically and is suitable for rapid sequential monitoring of dynamic processes.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA18-04902S" target="_blank" >GA18-04902S: Instrumentation for continuous on-line electtrophoretic monitoring of metabolic processes in living organisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Volume of the periodical

42

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

749-755

UT code for WoS article

000595222700001

EID of the result in the Scopus database

2-s2.0-85097026583