An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43920783" target="_blank" >RIV/00216208:11120/20:43920783 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/20:00552828

Result on the web

<a href="https://doi.org/10.1038/s41596-020-0401-x" target="_blank" >https://doi.org/10.1038/s41596-020-0401-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41596-020-0401-x" target="_blank" >10.1038/s41596-020-0401-x</a>

Result language

angličtina

Original language name

An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity

Original language description

This optimized protocol (including links to instruction videos) describes a comet-based in vitro DNA repair assay that is relatively simple, versatile, and inexpensive, enabling the detection of base and nucleotide excision repair activity. Protein extracts from samples are incubated with agarose-embedded substrate nucleoids (&apos;naked&apos; supercoiled DNA) containing specifically induced DNA lesions (e.g., resulting from oxidation, UVC radiation or benzo[a]pyrene-diol epoxide treatment). DNA incisions produced during the incubation reaction are quantified as strand breaks after electrophoresis, reflecting the extract&apos;s incision activity. The method has been applied in cell culture model systems, human biomonitoring and clinical investigations, and animal studies, using isolated blood cells and various solid tissues. Once extracts and substrates are prepared, the assay can be completed within 2 d.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/GA19-10543S" target="_blank" >GA19-10543S: Changes in DNA repair system associated with therapy outcome of ovarian carcinoma.</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Protocols

ISSN

1754-2189

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

35

Pages from-to

3844-3878

UT code for WoS article

000590016600002

EID of the result in the Scopus database

2-s2.0-85096054339