An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F20%3A00552828" target="_blank" >RIV/68378041:_____/20:00552828 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/20:43920783
Result on the web
<a href="https://www.nature.com/articles/s41596-020-0401-x" target="_blank" >https://www.nature.com/articles/s41596-020-0401-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41596-020-0401-x" target="_blank" >10.1038/s41596-020-0401-x</a>
Alternative languages
Result language
angličtina
Original language name
An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity
Original language description
This optimized protocol (including links to instruction videos) describes a comet-based in vitro DNA repair assay that is relatively simple, versatile, and inexpensive, enabling the detection of base and nucleotide excision repair activity. Protein extracts from samples are incubated with agarose-embedded substrate nucleoids ('naked' supercoiled DNA) containing specifically induced DNA lesions (e.g., resulting from oxidation, UVC radiation or benzo[a]pyrene-diol epoxide treatment). DNA incisions produced during the incubation reaction are quantified as strand breaks after electrophoresis, reflecting the extract's incision activity. The method has been applied in cell culture model systems, human biomonitoring and clinical investigations, and animal studies, using isolated blood cells and various solid tissues. Once extracts and substrates are prepared, the assay can be completed within 2 d.nnThis protocol describes a comet-based in vitro assay for detecting base and nucleotide excision repair activity for use in cell culture model systems, human biomonitoring and clinical investigations, and animal studies, using isolated blood cells and various solid tissues.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30101 - Human genetics
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Protocols
ISSN
1754-2189
e-ISSN
1750-2799
Volume of the periodical
15
Issue of the periodical within the volume
12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
35
Pages from-to
3844-3878
UT code for WoS article
000590016600002
EID of the result in the Scopus database
2-s2.0-85096054339