Low noncarbonic buffer power amplifies acute respiratory acid-base disorders in patients with sepsis: an in vitro study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F21%3A43921730" target="_blank" >RIV/00216208:11120/21:43921730 - isvavai.cz</a>

Alternative codes found

RIV/00064173:_____/21:N0000049

Result on the web

<a href="https://doi.org/10.1152/japplphysiol.00787.2020" target="_blank" >https://doi.org/10.1152/japplphysiol.00787.2020</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1152/japplphysiol.00787.2020" target="_blank" >10.1152/japplphysiol.00787.2020</a>

Result language

angličtina

Original language name

Low noncarbonic buffer power amplifies acute respiratory acid-base disorders in patients with sepsis: an in vitro study

Original language description

RATIONALE: Septic patients have typically reduced concentrations of hemoglobin and albumin, the major components of non-carbonic buffer power(β). This could expose patients to high pH variations during acid-base disorders. OBJECTIVES: To compare, in-vitro, non-carbonic β of septic patients with that of healthy volunteers, and evaluate its distinct components. METHODS: Whole blood and isolated plasma of 18 septic patients and 18 controls were equilibrated with different CO(2) mixtures. Blood gases, pH and electrolytes were measured. Non-carbonic β and non-carbonic β due to variations in Strong Ion Difference (β(SID)) were calculated for whole blood. Non-carbonic β and non-carbonic β normalized for albumin concentrations (β(NORM)) were calculated for isolated plasma. Representative values at pH=7.40 were compared. Albumin proteoforms were evaluated via two-dimensional electrophoresis. MEASUREMENTS AND MAIN RESULTS: Hemoglobin and albumin concentrations were significantly lower in septic patients. Septic patients had lower non-carbonic β both of whole blood (22.0+-1.9 vs. 31.6+-2.1 mmol/L, p&lt;0.01) and plasma (0.5+-1.0 vs. 3.7+-0.8 mmol/L, p&lt;0.01). Non-carbonic β(SID) was lower in patients (16.8+-1.9 vs. 24.4+-1.9 mmol/L, p&lt;0.01) and strongly correlated with hemoglobin concentration (r=0.94, p&lt;0.01). Non-carbonic β(NORM) was lower in patients (0.01 [-0.01 - 0.04] vs. 0.08 [0.06 - 0.09] mmol/g, p&lt;0.01). Septic patients and controls showed different amounts of albumin proteoforms. CONCLUSIONS: Septic patients are exposed to higher pH variations for any given change in CO(2) due to lower concentrations of non-carbonic buffers and, possibly, an altered buffering function of albumin. In both septic patients and healthy controls, electrolyte shifts are the major buffering mechanism during respiratory acid-base disorders.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30221 - Critical care medicine and Emergency medicine

Project

—

Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Physiology

ISSN

8750-7587

e-ISSN

—

Volume of the periodical

131

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

464-473

UT code for WoS article

000693665100004

EID of the result in the Scopus database

2-s2.0-85113590461