Secretory carrier-associated membrane protein 5 regulates cell-surface targeting of T-type calcium channels

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F23%3A43925832" target="_blank" >RIV/00216208:11120/23:43925832 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1080/19336950.2023.2230776" target="_blank" >https://doi.org/10.1080/19336950.2023.2230776</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/19336950.2023.2230776" target="_blank" >10.1080/19336950.2023.2230776</a>

Result language
angličtina
Original language name
Secretory carrier-associated membrane protein 5 regulates cell-surface targeting of T-type calcium channels
Original language description
Missense mutations in the human secretary carrier-associated membrane protein 5 (SCAMP5) cause a variety of neurological disorders including neurodevelopmental delay, epilepsy, and Parkinson's disease. We recently documented the importance of SCAMP2 in the regulation of T-type calcium channel expression in the plasma membrane. Here, we show that similar to SCAMP2, the co-expression of SCAMP5 in tsA-201 cells expressing recombinant Ca(v)3.1, Ca(v)3.2, and Ca(v)3.3 channels nearly abolished whole-cell T-type currents. Recording of intramembrane charge movements revealed that SCAMP5-induced inhibition of T-type currents is primarily caused by the reduced expression of functional channels in the plasma membrane. Moreover, we show that SCAMP5-mediated downregulation of Ca(v)3.2 channels is essentially preserved with disease-causing SCAMP5 R91W and G180W mutations. Hence, this study extends our previous findings with SCAMP2 and indicates that SCAMP5 also contributes to repressing the expression of T-type channels in the plasma membrane.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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