Cooperative roles of glucose and asparagine-linked glycosylation in T-type calcium channel expression

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00469487" target="_blank" >RIV/61388963:_____/16:00469487 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00424-016-1881-y" target="_blank" >http://dx.doi.org/10.1007/s00424-016-1881-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00424-016-1881-y" target="_blank" >10.1007/s00424-016-1881-y</a>

Result language

angličtina

Original language name

Cooperative roles of glucose and asparagine-linked glycosylation in T-type calcium channel expression

Original language description

T-type calcium channels are key contributors to neuronal physiology where they shape electrical activity of nerve cells and contribute to the release of neurotransmitters. Enhanced T-type channel expression has been causally linked to a number of pathological conditions including peripheral painful diabetic neuropathy. Recently, it was demonstrated that asparagine-linked glycosylation not only plays an essential role in regulating cell surface expression of Ca(v)3.2 channels, but may also support glucose-dependent potentiation of T-type currents. However, the underlying mechanisms by which N-glycosylation and glucose levels modulate the expression of T-type channels remain elusive. In the present study, we show that site-specific N-glycosylation of Ca(v)3.2 is essential to stabilize expression of the channel at the plasma membrane. In contrast, elevated external glucose concentration appears to potentiate intracellular forward trafficking of the channel to the cell surface, resulting in an increased steady-state expression of the channel protein at the plasma membrane. Collectively, our study indicates that glucose and N-glycosylation act in concert to control the expression of Ca(v)3.2 channels, and that alteration of these mechanisms may contribute to the altered expression of T-type channels in pathological conditions.

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pflugers Archiv - European Journal of Physiology

ISSN

0031-6768

e-ISSN

—

Volume of the periodical

468

Issue of the periodical within the volume

11/12

Country of publishing house

DE - GERMANY

Number of pages

15

Pages from-to

1837-1851

UT code for WoS article

000389834100005

EID of the result in the Scopus database

2-s2.0-84988723398