Modulation of Ca(v)3.2 T-type calcium channel permeability by asparagine-linked glycosylation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00461232" target="_blank" >RIV/61388963:_____/16:00461232 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/19336950.2016.1138189" target="_blank" >http://dx.doi.org/10.1080/19336950.2016.1138189</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/19336950.2016.1138189" target="_blank" >10.1080/19336950.2016.1138189</a>
Alternative languages
Result language
angličtina
Original language name
Modulation of Ca(v)3.2 T-type calcium channel permeability by asparagine-linked glycosylation
Original language description
Low-voltage-gated T-type calcium channels are expressed throughout the nervous system where they play an essential role in shaping neuronal excitability. Defects in T-type channel expression have been linked to various neuronal disorders including neuropathic pain and epilepsy. Currently, little is known about the cellular mechanisms controlling the expression and function of T-type channels. Asparagine-linked glycosylation has recently emerged as an essential signaling pathway by which the cellular environment can control expression of T-type channels. However, the role of N-glycans in the conducting function of T-type channels remains elusive. In the present study, we used human Ca(v)3.2 glycosylation-deficient channels to assess the role of N-glycosylation on the gating of the channel. Patch-clamp recordings of gating currents revealed that N-glycans attached to hCa(v)3.2 channels have a minimal effect on the functioning of the channel voltage-sensor. In contrast, N-glycosylation on specific asparagine residues may have an essential role in the conducting function of the channel by enhancing the channel permeability and / or the pore opening of the channel. Our data suggest that modulation of N-linked glycosylation of hCa(v)3.2 channels may play an important physiological role, and could also support the alteration of T-type currents observed in disease states.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Channels
ISSN
1933-6950
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
175-184
UT code for WoS article
000374581700004
EID of the result in the Scopus database
2-s2.0-84978393937