A new coaxial flow-through probe for electromembrane extraction of methadone from clinical samples on-line coupled to capillary electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F24%3A43926867" target="_blank" >RIV/00216208:11120/24:43926867 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10478700
Result on the web
<a href="https://doi.org/10.1016/j.aca.2024.342461" target="_blank" >https://doi.org/10.1016/j.aca.2024.342461</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2024.342461" target="_blank" >10.1016/j.aca.2024.342461</a>
Alternative languages
Result language
angličtina
Original language name
A new coaxial flow-through probe for electromembrane extraction of methadone from clinical samples on-line coupled to capillary electrophoresis
Original language description
Background: A new design of a flow-through coaxial electromembrane extraction (EME) probe that can be on-line coupled with CE instrument is described and tested. The supporting base of the probe is a PDMS microchip with T-shaped channels into which two coaxially arranged capillaries for inlet and outlet solutions are inserted. The extraction part of the probe is a porous polypropylene hollow fiber, sealed at one end and modified with nitrophenyloctyl ether (NPOE) extraction fluid. The internal volume of the extraction probe is 1.1 μL. Results: The EME probe was tested on laboratory samples and methadone was extracted into 3.0 M AcOH as acceptor. The concentration dependence was linear in the range of 0.1-1.0 μg mL-1 at EME 300 s/150 V and in the range of 0.5-10.0 μg mL-1 at EME 100 s/150 V. The enrichment factor was greater than 30 and the LOD was 0.21 μg mL-1. The EME of methadone in clinical samples showed a linear concentration dependence in human urine and a nonlinear concentration dependence in serum. The distribution of methadone in each phase of the extraction system and the effect of extraction membrane thickness on the enrichment factor were studied. The EME probe can be applied repeatedly. Significance: The supporting base of EME probe and flow gating interface (FGI) are realized by a microfluidic PDMS microchips cast in the laboratory without the use of lithography. A supporting PDMS chip with coaxially arranged capillaries and extraction membrane forms a compact analytical instrument. The entire EME/CE analysis process is performed on a laboratory-made instrument and automated by LabView.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-22398S" target="_blank" >GA22-22398S: Microfluidic and electronic devices for on-line electrophoretic analysis of adipose tissue</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
1873-4324
Volume of the periodical
1300
Issue of the periodical within the volume
April
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
342461
UT code for WoS article
001209254500001
EID of the result in the Scopus database
2-s2.0-85187199552