β-hydroxybutyrate exposure restores mitochondrial function in skeletal muscle satellite cells of critically ill patients
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F24%3A43926976" target="_blank" >RIV/00216208:11120/24:43926976 - isvavai.cz</a>
Alternative codes found
RIV/00064173:_____/24:43926976
Result on the web
<a href="https://doi.org/10.1016/j.clnu.2024.04.009" target="_blank" >https://doi.org/10.1016/j.clnu.2024.04.009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.clnu.2024.04.009" target="_blank" >10.1016/j.clnu.2024.04.009</a>
Alternative languages
Result language
angličtina
Original language name
β-hydroxybutyrate exposure restores mitochondrial function in skeletal muscle satellite cells of critically ill patients
Original language description
Background & aim: Dysfunction of skeletal muscle satellite cells might impair muscle regeneration and prolong ICU-acquired weakness, a condition associated with disability and delayed death. This study aimed to elucidate the distinct metabolic effects of critical illness and β-OH-butyrate on satellite cells isolated from these patients. Methods: Satellite cells were extracted from vastus lateralis muscle biopsies of patients with ICU-acquired weakness (n = 10) and control group of healthy volunteers or patients undergoing elective hip replacement surgery (n = 10). The cells were exposed to standard culture media supplemented with β-OH-butyrate to assess its influence on cell proliferation by ELISA, mitochondrial functions by extracellular flux analysis, electron transport chain complexes by high resolution respirometry, and ROS production by confocal microscopy. Results: Critical illness led to a decline in maximal respiratory capacity, ATP production and glycolytic capacity and increased ROS production in ICU patients' cells. Notably, the function of complex II was impaired due to critical illness but restored to normal levels upon exposure to β-OH-butyrate. While β-OH-butyrate significantly reduced ROS production in both control and ICU groups, it had no significant impact on global mitochondrial functions. Conclusion: Critical illness induces measurable bioenergetic dysfunction of skeletal muscle satellite cells. β-OH-butyrate displayed a potential in rectifying complex II dysfunction caused by critical illness and this warrants further exploration.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30221 - Critical care medicine and Emergency medicine
Result continuities
Project
<a href="/en/project/NU21J-06-00078" target="_blank" >NU21J-06-00078: Skeletal muscle regeneration in survivors of critical illness: How to prevent satellite cell failure?</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Nutrition
ISSN
0261-5614
e-ISSN
1532-1983
Volume of the periodical
43
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
1250-1260
UT code for WoS article
001292331900001
EID of the result in the Scopus database
2-s2.0-85190721391