The heteromeric GABA-B receptor recognizes G-protein alpha subunit C-termini

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F99%3A43903510" target="_blank" >RIV/00216208:11120/99:43903510 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/S0028-3908(99)00135-5" target="_blank" >http://dx.doi.org/10.1016/S0028-3908(99)00135-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/S0028-3908(99)00135-5" target="_blank" >10.1016/S0028-3908(99)00135-5</a>

Result language

angličtina

Original language name

The heteromeric GABA-B receptor recognizes G-protein alpha subunit C-termini

Original language description

The recently cloned GABA-B receptors are related to the metabotropic glutamate receptors (mGlu receptors), the Ca2+-sensing receptor and one group of vomeronasal receptors. The GABA-B receptors likely function in a heterodimeric form, constituted of GABA-BR1 and GABA-BR2. This novel feature in the G-protein coupled receptors (GPCRs) structure raises questions as to the mechanism of recognition of G-proteins by such receptors. In the present study we show that the GABA-BR1 and BR2 subunits form a functional receptor that recognizes the extreme C-termini of the G alpha i and G alpha o proteins when expressed in HEK293 cells. Indeed, heteromeric GABA-BR1/BR2 receptors do not activate PLC when co-expressed with G alpha q, but do so when co-expressed with the chimeric G alpha qi5 or G alpha qo5 subunits, the G alpha q subunit in which the 5 C-terminal residues are those of G alpha i or G alpha o, respectively. Interestingly, the heteromeric GABA-B receptor did not activate the chimeric G al

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

1999

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neuropharmacology

ISSN

0028-3908

e-ISSN

—

Volume of the periodical

38

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1657-1666

UT code for WoS article

000082959900004

EID of the result in the Scopus database

—