Effect of Culture Substrate and Culture Conditions on Lens Epithelial Cell Proliferation and alpha-smooth Muscle Actin Expression

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F09%3A5741" target="_blank" >RIV/00216208:11130/09:5741 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of Culture Substrate and Culture Conditions on Lens Epithelial Cell Proliferation and alpha-smooth Muscle Actin Expression

Original language description

The most common complication following cataract surgery is the posterior capsule opacification (PCO). It is the manifestation of migration and proliferation of lens epithelial cells (LEC). We used the primary and secondary culture of porcine LECs cultivated on collagen I, IV, glass, and uncoated plastic dishes. We studied cell proliferation, and expression of ? -SMA, and of alpha, beta, and gamma-crystallins. The effect of medium exchange schedule was studied using the TOTL-86 rabbit epithelial lens cell line. There was no difference in growth characteristics of primary cultures on different substrates. In secondary culture, LECs adhered better on the collagen surfaces. The culture substrate influenced the LEC proliferation and ?-SMA expression. The proliferation was enhanced when the medium was changed in comparison with an extra medium added on the 4th day. The cells did not synthesize ?- or ?-crystalline, the expression of ?-crystallin diminished.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5500

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

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UT code for WoS article

000265765100006

EID of the result in the Scopus database

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